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tetraspanin microarray chips  (NanoView Biosciences)

 
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    NanoView Biosciences tetraspanin microarray chips
    Tetraspanin Microarray Chips, supplied by NanoView Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tetraspanin+microarray+chips/exoview+tetraspanin+chips/10__1016_slash_j__vesic__2025__100066-70-1-19
    Average 90 stars, based on 1 article reviews
    tetraspanin microarray chips - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Exosomal delivery of IL-10: Biodistribution, pharmacokinetics, and preterm birth prevention strategies
    Article Snippet: This study investigates the potential of extracellular vesicles (EVs) loaded with interleukin-10 (IL-10) to reduce infection-induced preterm birth (PTB).. IL-10 has shown promise in reducing PTB by dampening inflammatory responses, but challenges exist with intraamniotic administration.. The study evaluates IL-10 gene-transfected cell-produced EVs containing IL-10 (tIL-10), comparing them with recombinant IL-10 (rIL-10) and rIL-10 incorporated in EVs via electroporation (eIL-10).



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    EVs are released by MUG-hOSEC model. EVs were detected by ExoView technology capturing the three exosome specific tetraspanins: CD9, CD63 and CD81 in three technical replicates. (a) Total fluorescence particle count of donor-derived EVs on tetraspanin capture spots and mouse IgG isotype control analyzed using fluorescence antibodies against CD9, CD63 and CD81. Presented is the Mean ± Standard Deviation (SD) of three technical replicates. (b) Size distribution of donor-derived EVs on tetraspanin capture spots analyzed using the ExoView200 software and are given as single dots for each tetraspanin capture. (c) Tetraspanin colocalization of donors using three fluorescent channels and overlay of fluorescent images, data show the tetraspanin colocalization of all detected EVs. Pie charts indicate the percentage of each tetraspanin colocalization on the released EVs in different colors for the three capturing spots, respectively; non coloc – indicates no colocalization. Concentration is given by particle counts per capture spot of each tetraspanin spot plus internal MIgG control, Mean ± Standard Deviation (SD). (d) Tetraspanin expression in EVs changes upon SDS treatment. EVs derived from skin punches treated with 10 µg/ml LPS or 1 % SDS were compared to EVs release by untreated skin punches of the same donor. Fold change in particle count is given for each of the three tetraspanin capture spots indicating changes in tetraspanin expression. (e) Size distribution of EVs derived from treated skin punches on tetraspanin capture spots analyzed using the ExoView200 software and are given as single dots for each tetraspanin capture.

    Journal: Scientific Reports

    Article Title: A human ex vivo skin model breaking boundaries

    doi: 10.1038/s41598-024-75291-7

    Figure Lengend Snippet: EVs are released by MUG-hOSEC model. EVs were detected by ExoView technology capturing the three exosome specific tetraspanins: CD9, CD63 and CD81 in three technical replicates. (a) Total fluorescence particle count of donor-derived EVs on tetraspanin capture spots and mouse IgG isotype control analyzed using fluorescence antibodies against CD9, CD63 and CD81. Presented is the Mean ± Standard Deviation (SD) of three technical replicates. (b) Size distribution of donor-derived EVs on tetraspanin capture spots analyzed using the ExoView200 software and are given as single dots for each tetraspanin capture. (c) Tetraspanin colocalization of donors using three fluorescent channels and overlay of fluorescent images, data show the tetraspanin colocalization of all detected EVs. Pie charts indicate the percentage of each tetraspanin colocalization on the released EVs in different colors for the three capturing spots, respectively; non coloc – indicates no colocalization. Concentration is given by particle counts per capture spot of each tetraspanin spot plus internal MIgG control, Mean ± Standard Deviation (SD). (d) Tetraspanin expression in EVs changes upon SDS treatment. EVs derived from skin punches treated with 10 µg/ml LPS or 1 % SDS were compared to EVs release by untreated skin punches of the same donor. Fold change in particle count is given for each of the three tetraspanin capture spots indicating changes in tetraspanin expression. (e) Size distribution of EVs derived from treated skin punches on tetraspanin capture spots analyzed using the ExoView200 software and are given as single dots for each tetraspanin capture.

    Article Snippet: Microarray chips coated with anti-tetraspanin antibodies against CD9, CD63, CD81 and mouse IgG as isotype control (Leprechaun Exosome Human Tetraspanin Kit 251–1044; LOT 032472001E and 032472001 F, Unchained Labs) were pre-scanned according to the manufacturer ́s instruction to generate a baseline before sample incubation.

    Techniques: Fluorescence, Derivative Assay, Control, Standard Deviation, Software, Capture-C, Concentration Assay, Expressing